Cholecystokinin and its COOH-terminal octapeptide in the pig brain.

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Cholecystokinin and its COOH-terminal octapeptide in the pig brain.

Two components--one resembling intact cholecystokinin in size and charge and immunologic specificity, and the other resembling the COOH-terminal octapeptide of cholecystokinin--have been found in extracts of the pig cerebral cortex. The relative concentrations of the two peptides in the extracts were dependent on the extractant, boiling 0.1 M HCl being more effective than boiling water for the ...

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Cholecystokinin-associated COOH-terminal peptides are fully sulfated in pig brain.

A radioimmunoassay was developed to detect the cholecystokinin (CCK)-associated nonapeptide (CAP-9) that forms the COOH terminus of pig preproCCK. This peptide (Ser-Ala-Glu-Glu-Tyr-Glu-Tyr-Thr-Ser) is presumably produced at the time that the tyrosine-sulfated octapeptide CCK8(s) is cleaved from preproCCK. Radioimmunoassay of a dried methanol extract of pig brain revealed no detectable CAP-9 imm...

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In comparison with a saline infusion, the infusion of the C-terminal octapeptide of cholecystokinin (4 ng/kg/min) decreased food intake by an average of 122 g in a group of 12 lean men without objective evidence of untoward side effects. Shapes of the cumulative intake curves under the two conditions were similar, but subjects ate less and stopped eating sooner when receiving octapeptide than w...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1977

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.74.7.3035